NR ATOY
AU Ziegler,U.; Weber,A.; Wagenschwanz,B.; Hoffmann,C.; Buschmann,A.; Groschup,M.H.
TI Experimental oral BSE infection of cattle - German Pathogenesis Study -2,5 years after infection
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-09
PT Konferenz-Poster
AB
Since January 2003 the Institut for Novel and Emerging Infectious Diseases is carrying out a pathogenesis study of BSE in cattle. A total of 56 calves (two groups of 28 animals each) was infected orally with a macerate of BSE positive brainstem (100g per animal). Another 18 animals received noninfected cattle brain stem homogenate to serve as mock controls.
All animals are bled and urine is taken every eight weeks as well as cerebrospinal fluid every four months (under sedation). In the course of serial kills, every four month 4-5 animals are euthanised and necropsied under TSE steril conditions to collect an extensive number of tissue and bodily fluid samples (i.e. more than 1.400 samples of more than 150 tissues from each euthanised animal).
Selected samples will subsequently be inoculated into transgenic mice, that overexpress the bovine PrP (Tgbov XV mice) and which are about 10.000 times more sensitive than wild type mice to cattle derived BSE prions. Using this novel bioassay, the spread of infectious agent from the alimentary tract to the CNS in bovines is analysed.
Immunohistological staining and immunoblotting examination of samples already demonstrated the accumulation of PrPsc in the distal ileum (Peyer's Patches) in the challenged animals, which were dissected 12 months p.i., 16 months p.i. and 20 months p.i. Therefore, the oral challenge was successful. Further immunochemical and immunochemical studies on the tissue samples more proximal to the CNS are currently under way.
Samples, which are taken at selected time points during the incubation period are of utmost importance for the development and the evaluation of new BSE diagnostic assays and in particular for the development of a live animal test.
IN
Seit 2003 läuft auf der Insel Riems die deutsche BSE-Pathogenesestudie. Man inokulierte 56 Kälber einer Herde oral mit jeweils 100 g Hirnhomogenat und fütterte zusätzlich noch 18 Rinder als Negativkontrolle mit der selben Menge Hirnhomogenat nicht BSE-infizierter Rinder. Den Tieren wird regelmäßig Blut und Urin entnommen und alle 4 Monate werden 4-5 getötet, um pro Tier mehr als 1400 Proben aus über 150 verschiedenen Geweben zu entnehmen. Mit diesen Proben werden transgene Mäuse intrazerebral inokuliert, die 10.000-fach empfänglicher als Wildtypmäuse sind.
Bisher konnte man bei 12, 16 und 20 Monate nach der oralen Inokulation getöteten Rindern immunohistologisch und per Western blot PrPsc in den Peyer-Platten des distalen Ileums nachweisen.
AD Ute Ziegler, Artur Weber, Baerbel Wagenschwanz, Chris Hoffmann, Anne Buschmann, Martin H. Groschup, Institute for Novel and Emerging Infectious Diseases at the Friedrich-Loeffler-Institute Federal Research Institute for Animal Health, Germany
SP englisch
PO Deutschland