NR ATPJ
AU Avrahami-Tzfati,D.; Hijazi,N.; Canello,T.; Tal,S.; Mincberg,M.; Gabizon,R.
TI Increased binding of PrPsc to cells infected with Mice minute virus (MVM)
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-20
PT Konferenz-Poster
AB Glycosaminoglycans (GAGs) such as heparan sulfate, are abundant polymers on the surface of mammalian cells and major components of the extracellular matrix. Various viruses, parasites and hormones (such as FGF) utilize cell surface heparan sulfate to enter target cells. Interestingly, FGF binding to cells has been shown to modulate virus infection, suggesting interaction between different GAGs ligands on the cell surface can modulate their activities. GAGs are also known to play important roles in prion replication such as the conversion of PrPc to PrPsc and PrPsc internalization and accumulation. To test whether other GAG ligands can modulate the binding of PrPsc to cells, we tested the binding of PrPsc to cells previously infected or uninfected, with Mice minute virus (MVM), a mouse parvovirus. We found a significantly higher incorporation of PrPsc into cells previously infected with the MVM parvovirus, as compared with the incorporation into uninfected cells. Higher incorporation was found not only in cells permissive to the virus, but also in non permissive cells, consistent with the possibility that increased PrPsc binding in the presence of virus is independent on viral replication. Interestingly, preliminary experiments suggest that the addition of the virus to ScN2A cells, a cell line permanently infected with prions, increased the accumulation of PrPsc in the cells, indicating a possible effect of the virus on additional GAG dependent steps of prion infection. Whether co-infection of prions with viruses or pre incubation with other GAGs ligands can accelerate prion infection in vivo remains to be investigated.
AD Dana Avrahami-Tzfati, Nuha Hijazi, Tamar Canello, Ruth Gabizon, Deparment of Neurology; The Agnes Ginges Center for Human Neurogenetics, Hadassah University Hospital, Jerusalem Israel; Saar Tal, Michal Mincberg, Department of Molecular Genetics of Development, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
SP englisch
PO Deutschland