NR ATQB
AU Seeger,H.; Heikenwälder,M.; Zeller,N.; Kranich,J.; Schwarz,P.; Gaspert,A.; Seifert,B.; Aguzzi,A.
TI Chronic kidney inflammation induces urinary prion excretion in scrapie-infected mice
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-38
PT Konferenz-Poster
AB Although prions are typically restricted to the central nervous and lymphoreticular system of infected hosts, chronic inflammation can expand the distribution of prion infectivity to unexpected sites. Here we tested whether various chronic inflammatory kidney disorders, in conjunction with prion infection, would trigger excretion of prion infectivity via urine. We found that urinary proteins from mice with organized lymphoid infiltrates and glomerulonephritis, taken at various preclinical and terminal stages of scrapie and injected intracerebrally into PrPc-overexpressing mice, induced PrPsc and clinical scrapie. In contrast, we were unable to detect urinary PrPsc or prionuria in prion-infected wild-type mice, in transgenic mice overexpressing PrPc in kidney, and in glomerulonephritic mice inoculated with non-infectious brain. Inflammatory conditions of excretory organs may therefore act as modifiers of natural prion transmission.
IN Weder bei nichtinfizierten, noch bei PrPc überexprimierenden oder "nur" Scrapie-infizierten Hausmäusen, aber bei Scrapie-infizierten Hausmäusen mit einer lymphozytischen Nierenentzündung fand man PrPsc und Scrapie-Infektiosität im Urin.
AD H.Seeger, M.Heikenwalder, N.Zeller, J.Kranich, P.Schwarz, A.Aguzzi, Institute of Neuropathology, University Hospital of Zürich, Switzerland; A.Gaspert, Institute of Clinical Pathology, University Hospital of Zürich, Switzerland; B.Seifert, Institute of of Biostatistics, University of Zürich, Switzerland
SP englisch
PO Deutschland