NR ATQG
AU Mouthon,F.; Chich,J.F.; Labas,V.; Picoli,C.; Bouin,A.P.; Schaeffer,B.; Deslys,J.P.; Grosclaude,J.
TI Cellular alterations during Prion infection : transcriptomic and proteomic approaches
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-43
PT Konferenz-Poster
AB
(F. Mouthon and J.F Chich equally contributed to this work)
The pathological hallmark of prion infected brain tissues are PrPres accumulation and neurodegeneration. Nevertheless, the cellular implications and molecular modifications involved in the pathogenesis and more widely during the cellular infection are still poorly understood. In order to identify cellular networks impaired by the infection, we have developped at the same time transcriptomic and proteomic approches on GT1-7 in vitro model of infection.
2-DE analysis for proteomics and Representational Difference Analysis coupled with cDNA Real Time PCR quantification for the transcriptomic approach, were performed on infected cell and as controls non-infected, non-infected treated (with a heparan sulfate mimetic drug), and infected-treated cells.
Proteomic approach allowed to find 60 spots (on 850-1000 detected spots) with variable expression pattern. All these spots were analysed by mass spectrometry to identify proteins presents in each of them.
For transcriptomic approach, the screening allowed to find 80 sequences disregulated in infected cells versus control out of which 44 were up-regulated and 36 were down-regulated.
In order to overcome the limitations linked to the specificity of a peculiar cell model or technique, we have organized (according to molecular fonctions and cellular components) the different modifications identified in order to define common metabolic pathways impaired by prion infection.
Deep alterations, in particular in macromolecules metabolism (DNA and proteins), in cellular growing and in molecular partners involved in oxydo-reduction mechanisms were observed by our transcriptomic and proteomic approaches.
Identification of molecular cascades involved at the cellular level should offer new insights for the comprehension of prion infection.
AD F.Mouthon, C.Picoli, J.P.Deslys, CEA/DSV/DRM/GIDTIP Route du panorama 92265 Fontenay-aux-Roses, France; J.F.Chich, J.Grosclaude, Biologie Physico-chimique des Prions Virologie et Immunologie Moléculaires INRA 78352 Jouy-en-Josas, France; B.Schaeffer, Biométrie et intelligence artificielle INRA 78352 Jouy-en-Josas, France; V.Labas, C.N.R.S. UMR 7637 E.S.P.C.I. 10 rue Vauquelin 75005 Paris, France; A.P.Bouin, DCMR CNRS Ecole Polytechnique 91128 Palaiseau, France
SP englisch
PO Deutschland
EA Bild 1, Bild 2, Bild 3, Ausschnitt 1, Ausschnitt 2, Ausschnitt 3, Ausschnitt 4