NR ATQH
AU Buschmann,A.; Groschup,M.H.
TI Distribution of BSE infectivity in a terminally BSE diseased cow is mainly restricted to the central and peripheral nervous system
QU International Conference - Prion 2005: Between fundamentals and society's needs - 19.10.-21.10.2005, Congress Center Düsseldorf - Poster Session: Pathogenesis PATH-44
PT Konferenz-Poster
AB The interspecies transmissibility of prion diseases is primarily influenced by the homology of the two involved prion proteins (PrPc). As revealed by several challenge experiments, transgenic mouse lines expressing a foreign PrPc display distinctly shorter incubation times in interspecies transmissions than nontransgenic mice. Here we describe the challenge of bovine PrPc transgenic mice (Tgbov XV mice) with various BSE homogenates. An endpoint titration in Tgbov XV mice and conventional RIII mice has revealed the superior sensitivity of Tgbov XV mice which exceeds that of RIII mice by at least 10.000. Tgbov XV mice were challenged with various tissues from a cow with end-stage clinical BSE. Results indicate that in cattle, BSE infectivity is restricted to the central and peripheral nervous system, while almost all examined lymphoid organs were free of BSE infectivity. The only lymphatic tissue containing infectivity were the Peyers patches from the distal ileum. Moreover, we found infectivity in the sciatic nerve and spurious amounts even in a sample of the Musculus semitendinosus. The BSE pathogenesis in cattle therefore varies fundamentally from that described for TSE infected sheep where large parts of the lymphoid tissue and other peripheral tissues contain infectivity from early after the infection.
IN Die Autoren verglichen mit Endpunkttitrationen die BSE-Empfänglichkeiten von RIII-Wildtypmäusen mit transgenen Tgbov XV-Mäusen und stellten fest, dass die transgenen Mäuse mit dem Rinderprionprotein mindestens 10.000 mal empfänglicher sind. Diese für Bioassays besonders geeigneten Mäuse inokulierten sie intracerebral mit verschiedenen Geweben einer einzelnen BSE-Kuh und fanden Infektiosität mit Ausnahme der Peyer-Platten des distalen Ileums ausschließlich in ZNS und peripheren Nerven sowie in Spuren auch im Musculus semitendinosus.
AD Anne Buschmann, Martin H. Groschup, Friedrich-Loeffler Institut, Greifswald - Insel Riems, Germany
SP englisch
PO Deutschland