NR ATUG
AU Nishida,N.; Katamine,S.; Manuelidis,L.
TI Reciprocal interference between specific CJD and scrapie agents in neural cell cultures
QU Science 2005 Oct 21; 310(5747): 493-6
PT journal article
AB Infection of mice with an attenuated Creutzfeldt-Jakob disease agent (SY-CJD) interferes with superinfection by a more virulent human-derived CJD agent (FU-CJD) and does not require pathological prion protein (PrPres). Using a rapid coculture system, we found that a neural cell line free of immune system cells similarly supported substantial CJD agent interference without PrPres. In addition, SY-CJD prevented superinfection by sheep-derived Chandler (Ch) and 22L scrapie agents. However, only 22L and not Ch prevented FU-CJD infection, even though both scrapie strains provoked abundant PrPres. This relationship between particular strains of sheep- and human-derived agents is likely to affect their prevalence and epidemic spread.
MH Animals; Cell Line; Coculture Techniques; *Creutzfeldt-Jakob Syndrome; Humans; Mice; Neurons/metabolism/*physiology; PrPsc Proteins/metabolism/*pathogenicity; Prions/metabolism/*pathogenicity/*physiology; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Scrapie; Sheep; Species Specificity; Virulence
AD Yale Medical School, New Haven, CT 06510, USA
SP englisch
PO USA