NR ATVN
AU Bolea,R.; Monleon,E.; Schiller,I.; Raeber,A.J.; Acin,C.; Monzon,M.; Martin-Burriel,I.; Struckmeyer,T.; Oesch,B.; Badiola,J.J.
TI Comparison of immunohistochemistry and two rapid tests for detection of abnormal prion protein in different brain regions of sheep with typical scrapie
QU Journal of Veterinary Diagnostic Investigation 2005 Sep; 17(5): 467-9
PT journal article
AB One of the "gold standard" techniques for postmortem confirmation of scrapie diagnosis in sheep and goats is immunohistochemical examination of brain tissue. Active surveillance for scrapie is mainly performed by rapid diagnostic tests on the basis of postmortem immunochemical detection of prion protein (PrP) in the obex tissue. The aim of this study was to determine the performance of 2 rapid tests, Prionics-Check LIA (a chemiluminescence sandwich enzyme-linked immunosorbent assay) and Prionics-Check Western blot for scrapie diagnosis when applied to brain areas other than the obex, in comparison with the recognized immunohistochemistry. Prion protein was detected in the obex, cervical spinal cord, and thalamus from all the scrapie-positive sheep by the 3 tests. Western blot and LIA were negative in other areas of the brain, although weak immunohistochemical staining was detected. The results show that the 2 rapid tests studied may detect PrP in brain areas other than the obex, although with a lower sensitivity than immunohistochemistry when there is minimal PrP deposition.
IN Im direkten Vergleich bei der Untersuchung verschiedener Hirnregionen von Schafen mit normalen Scrapie-Varianten stellten die Autoren fest, dass die immunhistochemische Untersuchung etwas sensitiver war als die bei Prionics-Schnelltests Prionics-Check LIA (chemiluminescence sandwich enzyme-linked immunosorbent assay) und Prionics-Check Western blot.
MH Animals; Blotting, Western/veterinary; Comparative Study; Enzyme-Linked Immunosorbent Assay/veterinary; Immunohistochemistry/veterinary; Prions/*isolation & purification; Research Support, Non-U.S. Gov't; Scrapie/diagnosis/*metabolism/pathology; *Sheep
AD National Reference Centre for TSE, Facultad de Veterinaria, Zaragoza, Spain.
SP englisch
PO USA