NR ATYB
AU Yull,H.M.; Ritchie,D.L.; Langeveld,J.P.M.; van Zijderveld,F.G.; Bruce,M.E.; Ironside,J.W.; Head,M.W.
TI Detection of type 1 prion protein in variant Creutzfeldt-Jakob disease
QU American Journal of Pathology 2006 Jan; 168(1): 151-7
PT journal article
AB Molecular typing of the abnormal form of the prion protein (PrPsc) has come to be regarded as a powerful tool in the investigation of the prion diseases. All evidence thus far presented indicates a single PrPsc molecular type in variant Creutzfeldt-Jakob disease (termed type 2B), presumably resulting from infection with a single strain of the agent (bovine spongiform encephalopathy). Here we show for the first time that the PrPsc that accumulates in the brain in variant Creutzfeldt-Jakob disease also contains a minority type 1 component. This minority type 1 PrPsc was found in all 21 cases of variant Creutzfeldt-Jakob disease tested, irrespective of brain region examined, and was also present in the variant Creutzfeldt-Jakob disease tonsil. The quantitative balance between PrPsc types was maintained when variant Creutzfeldt-Jakob disease was transmitted to wild-type mice and was also found in bovine spongiform encephalopathy cattle brain, indicating that the agent rather than the host specifies their relative representation. These results indicate that PrPsc molecular typing is based on quantitative rather than qualitative phenomena and point to a complex relationship between prion protein biochemistry, disease phenotype and agent strain.
MH Amino Acid Sequence; Animals; Antibodies, Monoclonal; Blotting, Western; *Brain Chemistry; Cattle; Comparative Study; Creutzfeldt-Jakob Syndrome/*metabolism; Encephalopathy, Bovine Spongiform/metabolism; Humans; Immunohistochemistry; Mice; Molecular Sequence Data; PrPsc Proteins/*chemistry/genetics; Research Support, Non-U.S. Gov't
AD National CJD Surveillance Unit, School of Molecular and Clinical Medicine, University of Edinburgh, Edinburgh, EH4 2XU, UK.; National CJD Surveillance Unit, Bryan Matthews Building, Western General Hospital, Edinburgh, EH4 2XU, UK. m.w.head@ed.ac.uk
SP englisch
PO USA