NR ATZA
AU Bate,C.; Boshuizen,R.S.; Williams,A.
TI Microglial cells kill prion-damaged neurons in vitro by a CD14-dependent process
QU Journal of Neuroimmunology 2005 Dec 30; 170(1-2): 62-70
PT journal article
AB Microglial cells killed primary cortical neurons exposed to the prion (PrP)-derived peptide HuPrP106-126. The survival of HuPrP106-126-damaged neurons was increased by pre-treating microglial cells with anti-CD14 antibodies, while microglial cells from CD14 knockout mice failed to kill HuPrP106-126-damaged neurons. In addition, HuPrP106-126-damaged neurons selectively bound a CD14-IgG chimera. The killing of HuPrP106-126-damaged neurons by human monocytes was epitope specific; it was reduced by pre-treating monocytes with some anti-CD14 monoclonal antibodies (mabs) (60bca, 3C10 or MAB3832), but not others (26ic or MAB3831). None of the mabs affected the survival of HuPrP106-126-damaged neurons in the absence of monocytes.
MH Animals; Antibodies/pharmacology; Antibodies, Monoclonal/pharmacology; Antigens, CD14/genetics/immunology/metabolism/*physiology; Cell Death; Cell Survival/drug effects; Cells, Cultured; Cerebral Cortex/*cytology; Coculture Techniques; Epitope Mapping; Epitopes; Humans; Immunoglobulin G/genetics; Mice; Mice, Inbred C57BL; Mice, Knockout; Microglia/*physiology; Monocytes/physiology; Neurons/drug effects/metabolism/*physiology; Peptide Fragments/pharmacology; Prions/pharmacology/*physiology; Recombinant Fusion Proteins/metabolism; Research Support, Non-U.S. Gov't
AD Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Herts AL9 7TA, UK. cbate@rvc.ac.uk
SP englisch
PO Niederlande