NR ATZO
AU Clinton,M.; Miele,G.; Nandi,S.; McBride,D.
TI Identification of disease markers by differential display: prion disease.
QU Methods in Molecular Biology 2006; 317: 157-78
PT journal article
AB In order to identify molecular markers of prion disease in peripheral tissues, we used the differential display reverse-transcriptase polymerase chain reaction (DDRT-PCR) procedure to compare gene expression in spleens of infected and uninfected mice. In this study, we identified a novel erythroid-specific gene that was differentially expressed as a result of prion infection. We were able to demonstrate that a decrease in the expression levels of this transcript in hematopoietic tissues was a common feature of prion diseases. Our findings suggest a previously unknown role for the blood erythroid lineage in the development of prion diseases and should provide a new focus for research into diagnostic and therapeutic strategies.
MH Animals; *Biological Markers; Blotting, Northern; Blotting, Southern; Cattle; Cloning, Molecular; DNA/chemistry; DNA, Complementary/metabolism; Electrophoresis, Agar Gel; Encephalopathy, Bovine Spongiform/diagnosis/metabolism; Erythroid Cells/cytology; Female; Gene Expression Profiling/*methods; Male; Mice; Mice, Inbred C57BL; Nucleic Acid Hybridization; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Prion Diseases/*diagnosis/*metabolism; RNA/chemistry; RNA, Messenger/metabolism; Research Support, Non-U.S. Gov't; Reverse Transcriptase Polymerase Chain Reaction; Spleen/metabolism; Tissue Distribution
AD Department of Gene Expression and Development, Roslin Institute, Scotland, UK
SP englisch
PO USA