NR ATZW
AU Dumpitak,C.; Beekes,M.; Weinmann,N.; Metzger,S.; Winklhofer,K.F.; Tatzelt,J.; Riesner,D.
TI The polysaccharide scaffold of PrP 27-30 is a common compound of natural prions and consists of alpha-linked polyglucose
QU Biological Chemistry 2005 Nov; 386(11): 1149-55
PT journal article
AB An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some branching via 1,4,6-linked glucose. We show that this polysaccharide scaffold is a common secondary component of prions found in hamster full-length PrPsc, prion rods and in mouse ScN2a prions from cell culture. The preparation from prion rods was improved, resulting in a polysaccharide scaffold free of remaining infectivity. Furthermore, we determined the stereochemistry of the glycoside linkages as pre-dominantly if not entirely alpha-glycosidic. The origin of the polysaccharide, its interaction with PrP and its potential relation to glycogen and corpora amylacea are discussed.
MH Animals; Cells, Cultured; Cricetinae; Glucans/*chemistry; Glycosylation; Mice; Molecular Structure; PrP 27-30 Protein/*chemistry; Prions/*chemistry; Research Support, Non-U.S. Gov't; Stereoisomerism
AD Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany.
SP englisch
PO Deutschland