NR AUBQ
AU Maximova,O.A.; Taffs,R.E.; Pomeroy,K.L.; Piccardo,P.; Asher,D.M.
TI Computerized morphometric analysis of pathological prion protein deposition in scrapie-infected hamster brain
QU Journal of Histochemistry and Cytochemistry 2006 Jan; 54(1): 97-107
PT journal article
AB Transmissible spongiform encephalopathies (TSEs or prion diseases) are characterized by a constellation of typical though variable pathological changes in the brain. Deposition of disease-associated abnormal prion protein (PrPsc) is the pathological feature of TSEs most consistent and accessible for quantification. However, the evaluation of PrPsc deposits detected by immunohistochemical techniques has been traditionally based on arbitrarily assigned semiquantitative scores. This approach is limited by its subjectivity and bias, yielding considerable variability. In this study, we used MetaMorph 6.1 image analysis software for quantitative analysis of immunostained PrPsc deposits in the CNS of hamsters infected with the 263K strain of scrapie agent. Computerized morphometric analysis (CMA) allowed unambiguous detection of even minimal amounts of immunostained PrPsc. CMA values for intensity of staining and area stained correlated well with semiquantitative scores, providing reproducible quantitative data and objective criteria for analyzing PrPsc deposition. CMA provides a simple and reliable method for improved and consistent diagnosis of TSEs that may also be used to quantify other immunostained biomarkers.
MH Animals; Brain/metabolism/*pathology; Cricetinae; Image Processing, Computer-Assisted; Immunohistochemistry; Internet; Mesocricetus; PrPsc Proteins/*metabolism; Scrapie/metabolism/*pathology
AD Division of Emerging and Transfusion-Transmitted Diseases, Center for Biologics Evaluation and Research, United States Food and Drug Administration, 1401 Rockville Pike, HFM-313 Rockville, Maryland 20852-1448, USA. maximova@cber.fda.gov
SP englisch
PO USA