NR AUFQ
AU Schwägele,F.
TI Information about BSE - Possibilities and limits of analysis
OT Informationen über BSE - Möglichkeiten und Grenzen der Analytik
QU Fleischwirtschaft 2001 Apr 9; 81(4): 143-5
PT article
AB Reliable procedures for the detection of components of cattle and tissue of the central nervous system (CNS) in meat products have gained significance with respect to BSE. Analytical methods for species identification are based either on protein or DNA, e.g. an immunological test like ELISA, polymerase chain reaction (PCR) in combination with analysis of restriction fragment length polymorphism (RFLP), hybridisation by means of DNA-probes (Southern-Blotting) and iso-electric-focusing (IEF) techniques. Concerning the different animal species in meat products the detection limits of the mentioned techniques are varying between several percent and traces. With the exception of IEF these methods record beef as well as other components originating from cattle in corresponding products. A test offered as a service by a private laboratory uses cholesterol, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) for the identification of CNS in meat products. Applying this method an animal species specific differentiation of CNS is not possible and the question is still open, if the method provides a tool to differentiate between CNS and peripheral nervous tissue in muscle.
SP deutsch
PO Deutschland