NR AUHY
AU Jouvin-Marche,E.; Attuil-Audenis,V.; Aude-Garcia,C.; Rachidi,W.; Zabel,M.; Podevin-Dimster,V.; Siret,C.; Huber,C.; Martinic,M.; Riondel,J.; Villiers,C.L.; Favier,A.; Naquet,P.; Cesbron,J.Y.; Marche,P.N.
TI Overexpression of cellular prion protein induces an antioxidant environment altering T cell development in the thymus
QU Journal of Immunology 2006 Mar 15; 176(6): 3490-7
PT journal article
AB Cellular prion protein (PrPc) is an ubiquitously expressed glycoprotein whose roles are still widely discussed, particularly in the field of immunology. Using TgA20- and Tg33-transgenic mice overexpressing PrPc, we investigated the consequences of this overexpression on T cell development. In both models, overexpression of PrPc induces strong alterations at different steps of T cell maturation. On TgA20 mice, we observed that these alterations are cell autonomous and lead to a decrease of alphabeta T cells and a concomitant increase of gammadelta T cell numbers. PrPc has been shown to bind and chelate copper and, interestingly, under a copper supplementation diet, TgA20 mice presented a partial restoration of the alphabeta T cell development, suggesting that PrPc overexpression, by chelating copper, generates an antioxidant context differentially impacting on alphabeta and gammadelta T cell lineage.
MH Animals; Antigens, CD/metabolism; Antioxidants/*metabolism; *Cell Differentiation; Copper/pharmacology; Dietary Supplements; Gene Expression Regulation; Mice; Mice, Inbred C57BL; Mice, Transgenic; Phenotype; Prions/genetics/*metabolism; Receptors, Antigen, T-Cell, alpha-beta/metabolism; Research Support, Non-U.S. Gov't; T-Lymphocytes/*cytology/drug effects/*metabolism; Thymus Gland/*cytology/*metabolism
AD Laboratoire d'Immunochimie, Commissariat a l'Energie Atomique, Institut National de la Sante et de la Recherche Medicale Unite 548, Universite J. Fourier, 17 Rue des Martyrs, 38054 Grenoble, France.
SP englisch
PO USA