NR AUMP
AU Holtbecker,J.N.
TI Detektion von bovinem Nervengewebe als Spezifiziertes Risikomaterial in Fleischerzeugnissen durch Etablierung eines speziesspezifischen ELISA für die Routinediagnostik unter Verwendung des Myelin Basic Protein
QU Inaugural-Dissertation zur Erlangung der tiermedizinischen Doktorwürde der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München von Judith Nathalie Holtbecker aus Münster, München, 23. Juli 2004
PT Dissertation
AB Some years ago, it was common practice to include brains of cattle and pigs in meat products. Shortly after the emergence of Bovine Spongiforme Encephalopathy, bovine brain was declared as specified risk material, due to the fact that, in case of an infection, pathogenic prions are mainly present in this organ. Test systems, which were thereupon developed fot the detection of central nervous system tissue in meat products did not allow the specific detection of bovine nerve tissue. One test system, even yielded false positive results for pork and poultry meat, a fact which was accompanied by commercial consequences for some producers of meat products. In this study, a method was generated for the species-specific detection of bovine nerve tissue in meat products. The method is an indirect ELISA, based on the detection of Myelin Basic Protein (MBP), using a monoclonal antibody against a continuous epitope. The detection of MBP is even possible after extended treatment at high temperatures, which is probably due to the continuous epitope. The detection limit was set at 0,1% bovine brain in scalding sausages, respectively meat products, with a cut-off of 0.500. Therefore, the MBP-ELISA is a sensitive method for the species-specific detection of bovine nerve tissue in meat products. Taking into consideration consumer protection aspects, the MBP-ELISA thus provides the means to control the absence of bovine brain in meat products.
SP deutsch
PO Deutschland