NR AUNT
AU Klein,F.; Lupo,T.; Pielack,D.; Mozola,M.
TI Validation study of a lateral-flow immunoassay for detection of ruminant by-product material in animal feeds and feed ingredients
QU Journal of AOAC International 2005 Nov-Dec; 88(6): 1583-92
PT journal article
AB An immunoassay with a lateral flow format has been developed for the detection of ruminant by-product material in animal feeds and feed ingredients. The test is designed for the analysis of animal feeds destined for feeding to ruminants to ensure that they do not contain ruminant by-products in violation of the ruminant-to-ruminant feed ban established by the U.S. Food and Drug Administration in 1997. This feed ban was established as a firewall against exposure of ruminant livestock animals to the prion agents responsible for neurological diseases such as bovine spongiform encephalopathy and scrapie. The test is designed for field use, e.g., at a feed mill, and yields a qualitative (presence/absence) result in 15-20 min. The objective of the study was to validate the lateral-flow test for detection of ruminant by-product material in a variety of finished animal feeds and feed ingredients. Results indicate that the test is specific for ruminant material and can detect as little as 1% ruminant material in these commodities.
MH *Animal Feed; Animals; Cattle; Chemistry, Analytical/methods; Chromatography/*methods; Food Contamination; Immunoassay/*methods; Prion Diseases/prevention & control; Prions/metabolism; Reference Standards; Reproducibility of Results; Ruminants; Sensitivity and Specificity
AD Neogen Corp., 620 Lesher P1, Lansing, MI 48912, USA
SP englisch
PO USA