NR AURE
AU Clark,S.J.; Baier,M.; Paley,R.; Hoffmann,R.W.; Oidtmann,B.
TI Expression profiles of duplicated prion proteins in fish
QU TSE-Forum, 6. Kongress - Nationale TSE-Forschungsplattform, Greifswald 26.6.-28.6.2006, Poster: Struktur und molekulare Mechanismen MOL-02
PT Konferenz-Poster
AB
We identified duplicated prion protein genes in a number of fish species (1, 2).
The presence of the normal cellular isoform of the prion protein (PrPc) is an absolute requirement for the replication of protease resistant prions (PrPsc). The identification of prion protein genes in fish poses the question whether fish might be susceptible to contract or transmit Transmissible Spongiform Encephalopathies (TSEs).
We had previously identified one Prion Protein gene in Common carp (Cyprinus carpio) and Rainbow trout (Oncorhynchus mykiss).
We now report identification of the duplicated PrP genes. Rainbow trout and Common carp are the most important freshwater species produced for human consumption in aquaculture in Europe.
We present real-time PCR data on the expression profiles of the duplicated PrP genes 1 and 2 of Carp (Cyprinus carpio). The investigation into which tissues fish PrPs are expressed in is vital. It allows to identify tissues which would be most prone to formation of the abnormal PrP isoform.
Presently, we are generating phage display derived monoclonal ABs against both PrPs found in the most important fish species produced for the table market. These will be used for a variety of purposes, including establishing protein expression profiles in various tissues, and in vitro conversion experiments.
Further work is fundamental to the identification of further homologues to mammalian PrP genes in fish and to a conclusive answer regarding the susceptibility of fish to contracting TSEs with the potential risk to us as a consumer.
1 Oidtmann B, Simon D, Holtkamp N, Hoffmann R, Baier M. (2003):
Identification of cDNAs from Japanese pufferfish (Fugu rubripes) and Atlantic salmon (Salmo salar) coding for homologues to tetrapod prion proteins.
FEBS Lett. 2003 538:96-100.
2 Rivera-Milla E, Oidtmann B, Panagiotidis CH, Baier M, Sklaviadis T, Hoffmann R, Zhou Y, Solis GP, Stuermer CA, Malaga-Trillo E. (2006): Disparate evolution of prion protein domains and the distinct origin of Doppel- and prion-related loci revealed by fish-to-mammal comparisons.
FASEB J 20: 317-319.
AD Sarah J. Clark, Rudolf W. Hoffmann, Institut für Zoologie, Fischbiologie und Fischkrankheiten, Kaulbachstr. 37, 80539 München; Michael Baier, Robert-Koch-Institut, Nordufer 20, 13353 Berlin; Richard Paley, Birgit Oidtmann, Cefas Weymouth Laboratory, Barrack Road, Weymouth, Dorset, DT4 8UB, UK
SP englisch
PO Deutschland
OR Tagungsband