NR AURH

AU Elfrink,K.; Salwierz,A.; Riesner,D.

TI Interaction of the Cellular Prion Protein with Raft-like Lipid Membranes

QU TSE-Forum, 6. Kongress - Nationale TSE-Forschungsplattform, Greifswald 26.6.-28.6.2006, Poster: Struktur und molekulare Mechanismen MOL-05

PT Konferenz-Poster

AB The conversion of the cellular isoform of the prion protein (PrPc) into the disease associated isoform (PrPsc) plays a key role in development of prion diseases. Within its cellular pathway PrPc undergoes several posttranslational modifications, i.e. the attachment of two N-linked glycans and a glycosyl phosphatidyl inositol (GPI)-anchor, by which it is linked to the plasma membrane on the exterior cell surface. Targeting the influence of the membrane environment on the conversion process we purified posttranslationally modified PrPc from transgenic chinese hamster ovary (CHO-) cells for in vitro studies (1, 2). The interaction of PrPc with model membranes was studied with both lipid vesicles in solution and lipid bilayers bound on a chip surface. The equilibrium and mechanism of PrPc binding to model membranes was analyzed by surface plasmon resonance. We could observe high specificity and affinity of PrPc to the model membrane. Depending on the degree of saturation of the membrane by PrPc the concentration of PrPc released from the membrane into the aqueous solution was estimated to be between 10-9 and 10-7 M. This corresponds to a free energy of the insertion reaction of -48 kJ/mol. Additionally, by specific reconstitution of PrPc into model membranes we established the basis for further analysis with respect to the influence of the membrane environment on PrP-conversion induced by infectious PrPsc-particles.
(1) Blochberger, T. C., Cooper, C., Peretz, D., Tatzelt, J., Griffith, O. H., Baldwin, M. A., Prusiner, S. B. (1997). Prion protein expression in Chinese hamster ovary cells using a glutamine synthetase selection and amplification system. Prot. Eng. 10, 1465-1473
(2) Elfrink, K., Riesner, D. (2004). Techniques in Prion Research. ed. by S. Lehmann and J. Grassi (Birkhäuser Verlag Basel), 4-15

AD Kerstin Elfrink, Agnieszka Salwierz and Detlev Riesner, Institut für Physikalische Biologie, Heinrich-Heine-Universität, Düsseldorf

SP englisch

PO Deutschland

EA Übersicht, oben, unten

OR Tagungsband

Autorenindex - authors index
Startseite - home page