NR AUUV
AU Garcia-Crespo,D.; Juste,R.A.; Hurtado,A.
TI Differential gene expression in central nervous system tissues of sheep with natural scrapie
QU Brain Research 2006 Feb 16; 1073-1074: 88-92
PT journal article
AB The expression of nine genes was analyzed by real-time RT-PCR in the central nervous system in order to investigate the molecular pathogenesis of natural scrapie. An up-regulation of genes related to glial activation (GFAP) and apoptosis (CASP3) was detected in obex and cerebrum, indicating a reactive glia. Another glial activation-related gene (CTSS) was slightly up-regulated in obex, whereas constitutive expression was detected for SOD1, YWHAZ, PRNP, and the apoptosis-related genes BCL2, MCL1, and BAX. This differential gene expression might reflect a spatial-temporal and tissue-specific molecular pathogenesis of scrapie.
MH Amyloid/genetics/metabolism; Animals; Caspases/genetics/metabolism; Central Nervous System/*metabolism/pathology; Comparative Study; Fibroblast Growth Factor 9/genetics/metabolism; Gene Expression/*physiology; Gene Expression Regulation/*physiology; Glial Fibrillary Acidic Protein/genetics/metabolism; Molecular Chaperones/genetics/metabolism; Protein Precursors/genetics/metabolism; RNA, Messenger/metabolism; Research Support, Non-U.S. Gov't; Reverse Transcriptase Polymerase Chain Reaction/methods; Scrapie/*metabolism; Sheep; Statistics, Nonparametric; Superoxide Dismutase/genetics/metabolism
AD Department of Animal Health, Instituto Vasco de Investigacion y Desarrollo Agrario (NEIKER); Berreaga, 1. 48160 Derio, Bizkaia, Spain.
SP englisch
PO Niederlande