NR AUWI
AU Wang,X.; Wang,F.; Arterburn,L.; Wollmann,R.; Ma,J.
TI The interaction between cytoplasmic prion protein and the hydrophobic lipid core of membrane correlates with neurotoxicity
QU The Journal of Biological Chemistry 2006 May 12; 281(19): 13559-65
PT journal article
AB Prion protein (PrP), normally a cell surface protein, has been detected in the cytosol of a subset of neurons. The appearance of PrP in the cytosol could result from either retro-translocation of misfolded PrP from the endoplasmic reticulum (ER) or impaired import of PrP into the ER. Transgenic mice expressing cytoplasmic PrP (cyPrP) developed neurodegeneration in cerebellar granular neurons, although no detectable pathology was observed in other brain regions. In order to understand why granular neurons in the cerebellum were most susceptible to cyPrP-induced degeneration, we investigated the subcellular localization of cyPrP. Interestingly, we found that cyPrP is membrane-bound. In transfected cells, it binds to the ER and plasma/endocytic vesicular membranes. In transgenic mice, it is associated with synaptic and microsomal membranes. Furthermore, the cerebellar neurodegeneration in transgenic mice correlates with the interaction between cyPrP and the hydrophobic lipid core of the membrane but not with either the aggregation status or the dosage of cyPrP. These results suggest that lipid membrane perturbation could be a cellular mechanism for cyPrP-induced neurotoxicity and explain the seemingly conflicting results concerning cyPrP.
MH Animals; Brain/cytology/metabolism; Cell Line; Cell Membrane/*chemistry/*metabolism; Cytoplasm/*metabolism; Gene Expression Regulation; Hydrophobicity; Lipid Bilayers/*chemistry/*metabolism; Mice; Mice, Transgenic; Neurons/metabolism/*pathology; Prions/*metabolism/*toxicity; Protein Binding; Research Support, Non-U.S. Gov't
AD Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, Ohio 43210, USA
SP englisch
PO USA