NR AVCF
AU Bach,S.; Tribouillard,D.; Talarek,N.; Desban,N.; Gug,F.; Galons,H.; Blondel,M.
TI A yeast-based assay to isolate drugs active against mammalian prions
QU Methods (San Diego, Calif.) 2006 May; 39(1): 72-7
PT journal article
AB Recently, we have developed a yeast-based (Saccharomyces cerevisiae) assay to isolate drugs active against mammalian prions. The initial assumption was that mechanisms controlling prion appearance and/or propagation could be conserved from yeast to human, as it is the case for most of the major cell biology regulatory mechanisms. Indeed, the vast majority of drugs we isolated as active against both [PSI(+)] and [URE3] budding yeast prions turned out to be also active against mammalian prion in three different mammalian cell-based assays. These results strongly argue in favor of common prion controlling mechanisms conserved in eukaryotes, thus validating our yeast-based assay and also the use of budding yeast to identify antiprion compounds and to study the prion world.
MH Animals; Anti-Infective Agents/*pharmacology; Cell Line; Cell Line, Tumor; Drug Evaluation, Preclinical/methods; False Positive Reactions; Methyltransferases/antagonists & inhibitors/genetics/metabolism; PrPsc Proteins/antagonists & inhibitors/genetics/metabolism; Prions/*antagonists & inhibitors/genetics/metabolism; Research Support, Non-U.S. Gov't; Saccharomyces cerevisiae/*drug effects/genetics/metabolism; Saccharomyces cerevisiae Proteins/antagonists &; inhibitors/genetics/metabolism
AD CNRS, Station Biologique, UPS2682, Place G. Teissier, 29680 Roscoff, Bretagne, France.
SP englisch
PO USA