NR AVCK
AU Benkemoun,L.; Sabate,R.; Malato,L.; Dos Reis,S.; Dalstra,H.; Saupe,S.J.; Maddelein,M.L.
TI Methods for the in vivo and in vitro analysis of [Het-s] prion infectivity
QU Methods (San Diego, Calif.) 2006 May; 39(1): 61-7
PT journal article
AB Prions have been described in mammals and fungi. The [Het-s] infectious genetic element of the filamentous fungus Podospora anserina is the prion form of the HET-s protein. This protein is involved in the control of a cell death reaction termed heterokaryon incompatibility. The infectious form of HET-s corresponds to a self-perpetuating amyloid. The purpose of the present paper is to describe the techniques that can be used to analyse [Het-s] prion propagation in vivo and HET-s amyloid aggregation in vitro. In addition, we report several methods that can be used to infect Podospora with recombinant HET-s amyloid.
MH Amyloid/chemistry/ultrastructure; Circular Dichroism; Fungal Proteins/chemistry/genetics/*metabolism; Gene Transfer Techniques; Green Fluorescent Proteins/genetics/metabolism; Microscopy, Electron; Podospora/genetics/*metabolism; Prions/genetics/*metabolism/ultrastructure; Recombinant Fusion Proteins/genetics/metabolism; Research Support, Non-U.S. Gov't; Spectrometry, Fluorescence; Spores, Fungal/genetics/metabolism
AD Laboratoire de Genetique Moleculaire des Champignons, IBGC UMR CNRS 5095, Universite de Bordeaux 2, Bordeaux, France.
SP englisch
PO USA