NR AVEL
AU Sebestik,J.; Hlavacek,J.; Stibor,I.
TI Rational design and synthesis of a double-stranded DNA-binder library
QU Biopolymers 2006; 84(4): 400-7
PT journal article
AB Described is a computer-assisted rational design of a DNA-bis-intercalator peptide library. The peptide library of 250 members was prepared and the most powerful binder identified. A value of the binding constant is almost two orders of magnitude higher than that of starting building block-9-aminoacridine. The binder affinity found toward calf thymus DNA is 30-fold of that of human prion peptide 106-126.
MH Aminacrine/chemistry; Animals; Cattle; Combinatorial Chemistry Techniques; Comparative Study; Computer-Aided Design; DNA/*chemical synthesis/chemistry/*metabolism; DNA-Binding Proteins/chemistry/metabolism; Fluorescent Dyes/chemistry; *Gene Library; Humans; Intercalating Agents/chemistry; Models, Molecular; Peptide Fragments/metabolism; Prions/metabolism; Research Support, Non-U.S. Gov't; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thymus Gland/chemistry
AD Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 166 10 Prague 6, Czech Republic.
SP englisch
PO USA