NR AVEO
AU Smid,L.M.; Vovko,T.D.; Popovic,M.; Petric,A.; Kepe,V.; Barrio,J.R.; Vidmar,G.; Bresjanac,M.
TI The 2,6-disubstituted naphthalene derivative FDDNP labeling reliably predicts Congo red birefringence of protein deposits in brain sections of selected human neurodegenerative diseases
QU Brain Pathology 2006 Apr; 16(2): 124-30
PT evaluation studies; journal article
AB Deposition of conformationally altered proteins prominently characterizes pathogenesis and pathomorphology of a number of neurodegenerative disorders. 2-(1-{6-[(2-[F-18]fluoroethyl) (methyl)amino]-2-naphthyl} ethylidene) malononitrile ([F-18]FDDNP), a hydrophobic, viscosity-sensitive, solvent-sensitive, fluorescent imaging probe has been used with positron emission tomography to visualize brain pathology in the living brain of Alzheimer disease (AD) patients. Its non-radiofluorinated analog FDDNP was shown to label senile plaques and neurofibrillary tangles (NFTs) in brain tissue sections. This work aimed at evaluating FDDNP labeling of various protein deposits in fixed, paraffin-embedded brain tissue sections of selected neurodegenerative disorders: AD, cerebral amyloid angiopathy (CAA), transmissible spongiform encephalopathies, progressive supranuclear palsy (PSP), Pick disease (PiD), Parkinson disease, dementia with Lewy bodies, multiple system atrophy (MSA). Cerebral hypertensive vascular hyalinosis (HVH) was used as negative control. Significant agreement between amyloid histochemical properties and FDDNP labeling of the deposits was established. FDDNP labeling showed high positive predictive value for birefringence in senile plaques and NFTs in AD, prion plaques and amyloid deposits in CAA. No FDDNP labeled structures were observed in HVH, PSP, PiD or MSA tissue sections. Our findings may be of significant value for the detection of neuropathological aggregates with [F-18]FDDNP in some of these disorders in the living brain of human subjects.
MH Birefringence; Comparative Study; Congo Red/chemistry/diagnostic use; Fluorescent Dyes/chemistry/diagnostic use; Fluorine Radioisotopes/chemistry/*diagnostic use; Humans; *Molecular Probe Techniques; Naphthalenes/chemistry/*diagnostic use; Nerve Tissue Proteins/analysis/metabolism; Neurodegenerative Diseases/*diagnosis/metabolism/radionuclide imaging; Neurofibrillary Tangles/chemistry/*metabolism; Nitriles/chemistry/diagnostic use; Predictive Value of Tests; Reproducibility of Results; Research Support, Non-U.S. Gov't; Senile Plaques/chemistry/*metabolism; Sensitivity and Specificity
AD Laboratory for Neural Plasticity and Regeneration, Institute of Pathophysiology, University of Ljubljana, Ljubljana, Slovenia. lojze.smid.jr@mf.uni-lj.si
SP englisch
PO Schweiz