NR AVEP
AU Stanczak,P.; Luczkowski,M.; Juszczyk,P.; Grzonka,Z.; Kozlowski,H.
TI Interactions of Cu2+ ions with chicken prion tandem repeats
QU Dalton Transactions 2004 Jul 21(14): 2102-7
PT journal article
AB The potentiometric and spectroscopic (EPR, UV-Vis, CD) data have shown that the chicken prion hexa-repeat (Ac-His-Asn-Pro-Gly-Tyr-Pro-NH(2)) is a very specific ligand for Cu(2+) ions. The His imidazole is an anchoring binding site, then the adjacent amide nitrogen coordinates as a second donor. The presence of Pro at position 3 induces binding of phenolate oxygen as a third donor atom. The tridentate coordination dominates around physiological pH. Similar to human octapeptide fragments, chicken tandem repeats exhibit a cooperative effect in binding Cu(2+) ions, although chicken peptides are much less effective in metal ion coordination.
MH Amino Acid Sequence; Animals; Cations, Divalent/*metabolism; *Chickens; Copper/*metabolism; Dimethyl Sulfoxide; Humans; Ligands; Molecular Sequence Data; Peptides/chemistry/metabolism; Prions/*chemistry/*metabolism; *Repetitive Sequences, Amino Acid; Research Support, Non-U.S. Gov't; Solutions; Spectrum Analysis; Water
AD Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland.
SP englisch
PO England