NR AVGY
AU Vadrot,C.; Darbord,J.C.
TI Quantitative evaluation of prion inactivation comparing steam sterilization and chemical sterilants: proposed method for test standardization.
QU The Journal of Hospital Infection 2006 Oct; 64(2): 143-8
PT evaluation studies; journal article
AB Prions are notoriously resistant to inactivation. To prevent accidental transmission of variant Creutzfeldt-Jakob disease (vCJD), various decontamination procedures have been adopted for re-usable medical devices by the authorities of countries at risk. As the vCJD agent in humans has a wide tissue distribution, practical methods of prion decontamination urgently need to be standardized, as do other sterilization and disinfection procedures (European Committee for Standardization). This article proposes a method using a quantitative murine model, combining observations of the decrease in the infection rate, the increase in the incubation period and a simultaneously performed chemical protein fixation control. In terms of practical application, autoclaving at 134 degrees C for 18 min or 121 degrees C for 30 min, and 1N sodium hydroxide for 15 min reduced the transmission of infectivity by a factor of at least 10(6). Partial efficacy can also be identified by the methodology, particularly for liquid cold sterilants such as glutaraldehyde and peracetic acid solutions.
MH Animals; Cross Infection/*prevention & control; Disease Models, Animal; Disinfectants; Disinfection/*methods; Equipment Contamination/prevention & control; Heat; Humans; Infection Control/methods; Mice; Mice, Inbred C57BL; Prion Diseases/*prevention & control; Scrapie/prevention & control; Surgical Instruments/*microbiology
AD Paris V University, Central Pharmacy of Hospitals, Paris, France.
SP englisch
PO England