NR AVKW
AU Didier,A.; Dietrich,R.; Steffl,M.; Gareis,M.; Groschup,M.H.; Müller-Hellwig,S.; Märtlbauer,E.; Amselgruber,W.M.
TI Cellular prion protein in the bovine mammary gland is selectively expressed in active lactocytes
QU Journal of Histochemistry and Cytochemistry 2006 Nov; 54(11): 1255-61
PT journal article
AB The cellular prion protein (PrPc) is a highly conserved glycoprotein with a still enigmatic physiological function. It is mainly expressed in the central nervous system but accumulating data suggest that PrPc is also found in a broad spectrum of non-neuronal tissue. Here we investigated the cell-type-related PrPc expression in the bovine mammary gland by using immunohistochemistry (IHC), ELISA, Western blot, and real-time RT-PCR. Specific immunostaining of serial sections revealed that PrPc is selectively localized in mammary gland epithelial cells. Particularly strong expression was found at the basolateral surface of those cells showing active secretion. Results obtained by RT-PCR and ELISA complemented IHC findings. No correlation was found between the level of PrPc expression and other parameters such as age of the animals under study or stage of lactation.
IN Didier et al. berichteten 2006, dass sie immunhistochemisch das normale zelluläre Prionprotein in Milchdrüsen von Kühen selektiv in Epithelzellen nachweisen konnten. Besonders stark war die PrPc-Expression in aktiv Milch produzierenden Zellen und gestützt wurden die Befunde durch Realtime-PCR und ELISA. Dagegen fanden die Autoren keinen Zusammenhang zwischen der PrPc-Expression und dem Alter oder der Laktationsphase der Kühe.
MH Animals; Blotting, Western; Cattle/*metabolism; Enzyme-Linked Immunosorbent Assay; Epithelial Cells/metabolism; Female; Immunohistochemistry; *Lactation; Mammary Glands, Animal/cytology/*metabolism; Organ Specificity; PrPc Proteins/*biosynthesis; Research Support, Non-U.S. Gov't; Reverse Transcriptase Polymerase Chain Reaction
AD Anatomy and Physiology of Domestic Animals, University of Hohenheim, Fruwirthstrasse 35 70599, Stuttgart, Germany.
SP englisch
PO USA