NR AVMC
AU Lezmi,S.; Ronzon,F.; Bencsik,A.A.; Bedin,A.; Calavas,D.; Richard,Y.; Simon,S.; Grassi,J.; Baron,T.G.M.
TI PrP(d) accumulation in organs of ARQ/ARQ sheep experimentally infected with BSE by peripheral routes
QU Acta Biochimica Polonica 2006; 53(2): 399-405
PT journal article; research support, non-u.s. gov't
AB To study the pathogenesis of bovine spongiform encephalopathy infection in small ruminants, two Lacaune sheep with the AA136RR154QQ171 and one with the AA136RR154RR171 genotype for the prion protein, were inoculated with a brain homogenate from a French cattle BSE case by peripheral routes. Sheep with the ARQ/ARQ genotype are considered as susceptible to prion diseases contrary to those with the ARR/ARR genotype. The accumulation of disease-associated prion protein (PrP(d)) was analysed by biochemical and immunohistochemical methods. No PrP(d) accumulation was detected in samples from the ARR/ARR sheep 2 years post inoculation. In the two ARQ/ARQ sheep that had scrapie-like clinical symptoms, PrP(d) was found in the central, sympathetic and enteric nervous systems and in lymphoid organs. Remarkably, PrP(d) was also detected in some muscle types as well as in all peripheral nerves that had not been reported previously thus revealing a widespread distribution of BSE-associated PrP(d) in sheep tissues.
MH Animals; Blotting, Western; Brain/metabolism/pathology; Cattle; Central Nervous System/metabolism/pathology; Digestive System/metabolism/pathology; Encephalopathy, Bovine Spongiform/*metabolism/pathology; Genotype; Immunohistochemistry; Lymphatic System/metabolism/pathology; PrPsc Proteins/*analysis/genetics; Retina/metabolism/pathology; Scrapie/metabolism/pathology; Sheep
AD Stéphane Lezmi (s.lezmi@lyon.afssa.fr), Frédéric Ronzon, Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Alexandre Bedin, Didier Calavas, Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France; Yves Richard, Ecole Nationale Vétérinaire de Lyon, Marcy L'Etoile, France; Stéphanie Simon, Jacques Grassi, 3CEA, Service de Pharmacologie et d'Immunologie, Gif sur Yvette cedex, France
SP englisch
PO Polen