NR AVUO
AU Gonzalez,L.; Chianini,F.; Martin,S.; Siso,S.; Gibbard,L.; Reid,H.W.; Jeffrey,M.J.
TI Comparative titration of experimental ovine BSE infectivity in sheep and mice
QU Journal of General Virology 2007 Feb; 88(2): 714-7
PT journal article; research support, non-u.s. gov't
AB Titration studies of the infectivity of experimental bovine spongiform encephalopathy (BSE) in sheep are necessary to assess the risk for human health posed by the ovine infection relative to the original cattle disease. Here, a comparative titration was performed of sheep-passaged BSE infectivity in Romney sheep and RIII mice, by the intracerebral (i.c.) and i.c. plus intraperitoneal (i.p.) routes, respectively. The sheep-to-mouse species barrier was lower than anticipated, as similar titres were obtained for both sheep [1 x 10(5.4) (i.c.) ID(50) g(-1))] and mice [1 x 10(5.0) (i.c.+i.p.) ID(50) g(-1)]. Moreover, sheep of the ARR/ARR PrP genotype all succumbed to i.c. challenge with a 10(-3) dilution of 0.5 g of a brainstem pool from BSE-affected sheep, indicating that resistance to natural infection in sheep of this genotype must reside in some mechanism of peripheral pathogenesis.
IN Bei intrazerebraler Inokulation mit verdünntem Hirnstammhomogenat von einem BSE-infizierten Schaf fanden die Autoren sehr ähnliche Empfänglichkeiten von Schafen und Wildtyp-Mäusen. Außerdem ließen sich durch intrazerebrale Inokulation auch ARR/ARR-Schafe relativ leicht mit Schaf-passagiertem BSE infizieren.
MH Animals; Brain/metabolism/pathology; Cattle; Encephalopathy, Bovine Spongiform/pathology/*physiopathology/*transmission; Mice; Prions/genetics/*pathogenicity; Sheep; Sheep Diseases/pathology/*physiopathology/*transmission; Species Specificity
AD Lorenzo González (l.gonzalez@vla.defra.gsi.gov.uk), Stuart Martin, Sílvia Sisó (s.siso@vla.defra.gsi.gov.uk), Martin J. Jeffrey (m.jeffrey@vla.maff.gov.uk), VLA Lasswade Veterinary Laboratory, Pentlands Science Park, Bush Loan, Penicuik, Edinburgh EH26 OPZ, Scotland, UK
SP englisch
PO England