NR AVVK
AU Aarts,H.J.M.; el Bouw,M.; Buntjer,J.B.; Lenstra,J.A.; Van Raamsdonk,L.W.
TI Detection of bovine meat and bone meal in animal feed at a level of 0.1%.
QU Journal of AOAC International 2006 Nov-Dec; 89(6): 1443-6
PT journal article; research support, non-u.s. gov't
AB For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133 degrees C for 20 min, a contamination level of 0.1% was detected.
MH Animal Feed/*analysis; Animals; Bone and Bones/*chemistry; Cattle; Chickens; DNA/chemistry; Equipment Reuse; Meat/*analysis; Reverse Transcriptase Polymerase Chain Reaction; Sheep
AD Henk J.M. Aarts (henk.aarts@wur.nl), M. el Bouw, Leo W. D. van Raamsdonk, RIKILT-Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD Wageningen, The Netherlands; Jaap B. Buntjer, Keygene N.V., PO Box 216, 6700 AE Wageningen, The Netherlands; Johannes A. Lenstra, Utrecht University, Faculty of Veterinary Medicine, Yalelaan 8, 3584 CM Utrecht, The Netherlands
SP englisch
PO USA