NR AVWQ
AU Han,J.; Zhang,J.; Yao,H.; Wang,X.; Li,F.; Chen,L.; Gao,C.; Gao,J.; Nie,K.; Zhou,W.; Dong,X.
TI Study on interaction between microtubule associated protein tau and prion protein
QU Science in China. Series C, Life Sciences / Chinese Academy of Sciences 2006 Oct; 49(5): 473-9
PT journal article
AB Microtubule-associated protein tau is considered to play roles in many neurodegenerative diseases including some transmissible spongiform encephalopathies. To address the possible molecular linkage of prion protein (PrP) and tau, a GST-fusion segment of human tau covering the three-repeat region and various PrP segments was used in the tests of GST pull-down and immunoprecipitation. We found tau protein interacted with various style prion proteins such as native prion protein (PrPc) or protease-resistant isoform (PrPsc). Co-localization signals of tau and PrP were found in the CHO cell tranfected with both PrP and tau gene. The domain of interaction with tau was located at N-terminal of PrP (residues 23 to 91). The evidence of molecular interactions between PrP and tau protein highlights a potential role of tau in the biological function of PrP and the pathogenesis of TSEs.
MH Animals; Brain/metabolism; Cell Line; Gene Expression; Humans; Microtubules/*metabolism; Prions/genetics/*metabolism; Protein Binding; Recombinant Proteins/genetics/metabolism; tau Proteins/genetics/isolation & purification/*metabolism
AD State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
SP englisch
PO China