NR AVXK
AU Maddison,B.C.; Whitelam,G.C.; Gough,K.C.
TI Cellular prion protein in ovine milk
QU Biochemical and Biophysical Research Communications 2007 Feb 2; 353(1): 195-9
PT journal article
AB Cellular prion protein, PrPc, is essential for the development of prion diseases where it is considered to be a substrate for the formation of the disease-associated conformer, PrPsc. In sheep, PrPc is abundant in neuronal tissue and is also found at lower concentrations in a range of non-neuronal tissues, including mammary gland. Here, we demonstrate the presence of soluble PrPc in the non-cellular, non-lipid fraction of clarified ovine milk. Compared with brain-derived PrPc, ovine milk PrPc displays an increased electrophoretic mobility. Ovine milk PrPc is mainly present as three species that differ in the extent of their N-linked glycosylation, with glycoform profiles varying among animals. Similar PrPc species are also present in fresh and commercial homogenised/pasteurised bovine milk, with additional N-terminal PrPc fragments detectable in ruminant milk and commercial milk products.
MH Animals; Food Analysis; Milk/*chemistry; PrPc Proteins/*analysis/*chemistry; Sheep
AD ADAS UK, Department of Biology, University of Leicester, University Road, Leicester, LE1 7RH, UK
SP englisch
PO USA