NR AWAS
AU Acin,C.; Monleon,E.; Monzon,M.; Bolea,R.; Vargas,A.; Badiola,J.J.
TI Neuropathological and immunochemical findings of suspect atypical scrapie cases detected in the Spanish surveillance programme (2003-2005)
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-01
PT Konferenz-Poster
AB During the last 5 years, scrapie surveillance has been encouraged with the aim of detecting most of the scrapie-affected animals; eradicating the scrapie disease and improving the detection of BSE cases in sheep or goats. As a consequence of the increase in the number of analysis, the number of scrapie cases has also been incremented and atypical cases of scrapie have been described along Europe (E.g. Nor98 cases). The European Food Safety Authority has defined an atypical scrapie case as: samples mild positive to rapid Western Blot (high concentration of PK); clearly positive to modified Western Blot (low concentration of PK) and a low band of <12KDa and finally highly positive in the cerebellum and mildly positive in the brainstem by immunohistochemistry. A retrospective study has been done analyzing suspect atypical scrapie cases diagnosed from 2003 to 2005 by the National Reference centre for TSEs of Zaragoza. Following the EFSA recommendations and the immunochemical protocols developed by Gonzalez et al, the envisaged techniques have been developed: Vacuolar lesional profile; Reactivity of intracellular PrPd to N-terminal antibodies (epitope mapping) and phenotypic characterization by immunohistochemistry with the P4, 6H4, R145 antibodies; Modified Western Blot to determine de glycoform ratio and molecular weights. In addition, the entire PRNP gene coding region of the selected animals has been sequenced in order to visualize the present SNPs. The results obtained reveal the presence of atypical scrapie cases in our country. In addition, these cases could have been sub estimated because the surveillance applied was based on the analysis of the brain steam by rapid Western Blot.
AD Department of Animal Pathology. University of Zaragoza, Zaragoza, Spain. E-mail: crisacin@unizar.es
SP englisch
PO Italien