NR AWBA
AU An,S.S.A.; Welker,E.; Lim,K.T.; Oh,H.J.; Lee,B.S.; Joo,Y.R.; Schmerr,M.J.; Yokoyama,T.; Kim,S.Y.
TI Detection of PrPsc in plasma from sheep using a multimer detection system-3D
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-02
PT Konferenz-Poster
AB Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases that include bovine spongiform encephalopathy (BSE), scrapie in sheep and goats, and Creutzfeldt-Jakob disease (CJD) in humans. The putative agent is a host cell surface glycoprotein, the prion protein, that aggregates after misfolding. Reports of transmission of both scrapie and BSE by blood transfusion in sheep have raised concern for the safety of blood and blood products. This concern has heightened after cases of vCJD contracted from blood transfusions were reported. We have developed a system called Multimer Detection System (MDS), in which a specific epitope-overlapping antibody system is used to differentiate the aggregated PrPsc/PrPc (multimer) from PrPc (monomer). MDS detected the PrPsc in various infectious samples - brain homogenate, plasma spiked with brain containing PrPsc from mouse and plasmas from a diseased hamsters and sheep. The MDS system was improved when we change from a plate to magnetic bead format (MDS-3D). The MDS-3D worked optimally when the plasma sample, buffer, magnetic beads coated with capturing antibody and detection antibody were added simultaneously. This could reduce the assay time by eliminating several incubation steps. Using the MDS-3D system, plasma from diseased sheep could be easily differentiated from normal animals. The MDS-3D system could be applied to human samples and potentially ensure the safety of the blood and blood products.
AD S.S.A. An, K.T. Lim, H.J. Oh, B.S. Lee: PeopleBio Inc., Seoul, Korea; E. Welker: Hungarian Academy of Sciences, Budapest, Hungary; Y.R. Joo: Korean National Institute of Health/KCDC, Seoul, Korea; M.J. Schmerr: Ames Laboratory, Ames, USA; T. Yokoyama: National Institute of Animal Health, Tsukuba, Japan; S.Y. Kim: Seoul National University Medical School, Bundang, Korea. E-mail: seongaan@yahoo.com
SP englisch
PO Italien