NR AWBP
AU Barr,J.B.; Watson,M.; Harris,N.; Brown,D.A.; Head,M.; Ironside,J.; Fraser,J.R.; Barron,R.M.
TI SELDI-assisted identification of biomarkers for TSE diagnosis
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-06
PT Konferenz-Poster
AB Definitive tests for the detection of TSE disease are currently restricted to the presence of the protein PrPsc found in tissue biopsies. For a pre-clinical diagnostic test to be effective, early markers must be established which ideally can also be found in body fluids such as blood. Our approach to finding new surrogate markers uses differential protein expression profiling and SELDI-MS-TOF technology in brain samples from a well characterised murine scrapie model to establish a panel of markers for scrapie diagnosis. The murine model used in this experiment (intracerebrally injected with ME7scrapie isolate) displays severe pathology in the hippocampus where it was thought that the highest number of disease specific markers would be found. Many potential biomarker profiles were detected by this method, some in the early stages of disease before clinical signs were obvious. The profiles can be used collectively as a panel of markers without formal identification however, by identifying individual proteins we can also establish potential single markers and their role in TSE pathogenesis. We have purified and identified individual proteins using mass spectrometry including SELDI, and western blotting techniques. The identified proteins were then localised in brain sections using immunocytochemical techniques. Proteins associated with protein folding and oxidative stress have been identified. Other groups have previously highlighted the importance of these pathways in scrapie pathogenesis however the use of these proteins as a panel rather than individual biomarkers will increase the potential of establishing a definitive diagnostic test for TSE disease. Having established and identified markers using this technique in brain tissue we intend to further this work by examining other tissues and blood.
AD Janice B. Barr (janice.barr@bbsrc.ac.uk), Debbie A. Brown (debbie.brown@bbsrc.ac.uk), Janet R. Fraser (janet.fraser@bbsrc.ac.uk), Rona M. Barron (rona.barron@bbsrc.ac.uk), Institute for Animal Health, BBSRC and MRC, Neuropathogenesis Unit, Ogston Building, West Mains Road, Edinburgh EH9 3JF, Scotland, UK; M. Watson: Institute for Animal Health, Compton, UK; N. Harris: Ciphergen Biosystems, Fremont CA, USA; M. Head, J. Ironside: National CJD Surveillance Unit, Edinburgh,UK
SP englisch
PO Italien