NR AWBV
AU Bencsik,A.A.; Lezmi,S.; Baron,T.G.M.
TI Pet-Blot analysis contributes to BSE strain recognition in C57BL/6 mice
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-03
PT Konferenz-Poster
AB Identification of the strain of agent responsible for the bovine spongiform encephalopathy (BSE) can be made on histological basis, through the analysis of both distribution and intensity of brain vacuolar lesions after BSE transmission to mouse. Another useful way to distinguish the BSE agent from other prion strains is the study of the abnormal prion protein (PrPres) distribution. For that purpose, here, the Paraffin-Embedded-Tissue-blot (PET-blot) method was applied on brains from C57Bl/6 mice infected with cattle BSE, experimental sheep-BSE or Feline Spongiform Encephalopathy (FSE) from a cheetah. The PrPres distribution was comparable whatever the 3 BSE agent sources considered and was distinct from the PrPPres distribution in C57Bl/6 mice inoculated with a French scrapie isolate or with a mouse-adapted scrapie strain (C506M3). These data confirm a common origin of infectious agent responsible for the British and French cattle BSE. They also indicate that PET-blot method appears as a precise complementary tool in prion strain studies as it offers an easy and quick assessment of the PrPres mapping. The advantages and limits of the PET-Blot method are discussed and compared to other established and validated methods of strain typing.
AD Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Stéphane Lezmi (s.lezmi@lyon.afssa.fr), Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France
SP englisch
PO Italien