NR AWBW
AU Bencsik,A.A.; Baron,T.G.M.
TI BSE agent in PrP-ARR/ARR sheep infected by peripheral route: complete PrPd-IHC study and transmission to ovine TG mice
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-07
PT Konferenz-Poster
AB The possible transmission of bovine spongiform encephalopathy (BSE) agent to ovine species is now considered for several years and demonstrated experimentally. More recently, the demonstration was made that after intracerebral challenge, the BSE agent was able to infect sheep believed as genetically most resistant to classical scrapie (PrPARR/ARR genotype). Here, we report and describe in detail the disease associated prion protein (PrPd) immunohistochemical analysis of more than 160 pieces of the organs from a PrPARR/ARR genotype sheep infected with the BSE agent by peripheral route. As PrPd was detected in the brain in absence of any clinical symptoms, transmission studies were performed using a sensitive ovine transgenic mouse model (Tg(OvPrP4)) also acknowledged to be able to distinguish the BSE agent. Here we demonstrate that these PrPd deposits were associated with an infectious power. Besides, the occurrence of florid plaques in the infected transgenic mouse brains provided additional evidence for a link with the BSE agent. Altogether these results suggests interestingly that silent carriers of the BSE agent may exist among ARR homozygous sheep.
AD Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Stéphane Lezmi (s.lezmi@lyon.afssa.fr), Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France
SP englisch
PO Italien