NR AWBX
AU Bencsik,A.A.; Coleman,A.W.; Debeer,S.O.S.; Perron,H.; Moussa,A.
TI Amplified immunohistochemical detection of PrPsc in animal transmissible spongiform encephalopathies using streptomycin
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-07
PT Konferenz-Poster
AB Due to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt-Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. Here, we describe the use of streptomycin sulfate in IHC, providing a novel original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using different PrP antibodies.
AD AD Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Sabine O. S. Debeer (s.debeer@lyon.afssa.fr), Aly Moussa, Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France; Anthony W. Coleman, Institut de Biologie et de Chimie des Protéines, University Lyon 1, Lyon, France (AWC); Hervé Perron, BioMérieux, R&D Immunoassay and Proteomics Department, Marcy l'Etoile, France
SP englisch
PO Italien