NR AWCA
AU Bergström,A.L.; Lind,P.; Laursen,H.
TI PrPres deposition in sCJD and GSS patients visualized with paraffin embedded tissue blot and immunohistochemistry
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-08
PT Konferenz-Poster
AB The transmissible spongiform encephalopathies are characterized by vacuolization, gliosis and deposition of a misfolded and protease-resistant variant (PrPres) of the prion protein (PrPc) in the central nervous system. Here, immunohistochemistry (IHC) and paraffin embedded tissue blot (PETblot), performed with the anti-PrP MAbs 3F4 and KG9, were combined to study the deposition of protease resistant PrPsc in brain sections from patients with sporadic Creutzfeldt-Jakob disease (sCJD) and Gerstmann-Sträussler-Scheinker disease (GSS). Generally, there was a good correlation between the PrPres-depositions visualized with IHC and PET-blot. However, the PET-blot tended to show an increased sensitivity compared to IHC. A comparison of the appearance and morphology of the PrPres-depositions in selected regions of the brain in patients with sCJD and GSS is presented here.
AD A.-L. Bergström: The Neuropathology Laboratory, The University Hospital of Copenhagen, Blegdamsvej 9, Copenhagen, Denmark, and Department of Veterinary Diagnostics and Research, Danish Institute for Food and Veterinary Research, Bulowsvej 27, Copenhagen, Denmark; P. Lind: Department of Veterinary Diagnostics and Research, Danish Institute for Food and Veterinary Research, Bulowsvej 27, Copenhagen, Denmark; H. Laursen: The Neuropathology Laboratory, The University Hospital of Copenhagen, Blegdamsvej 9, Copenhagen, Denmark
SP englisch
PO Italien