NR AWCD
AU Bilheude,J.M.; Brun,A.; Morel,N.; Henaux,S.; Andreoletti,O.; Grassi,J.; Torres,J.M.
TI Characterization of the PrP polymorphism in position 171 by an ELISA test, application for the selection of sheep resistant to TSE
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions GEN-04
PT Konferenz-Poster
AB Scrapie is a transmissible spongiform encephalopathy (TSE) which naturally affects sheep and goat. In the last years, concerns have been raised concerning the possible presence of BSE in small ruminant flocks. Following Commission Decision 2003/100/EC many countries have to introduce a breeding program to select for resistance to TSEs in sheep herds based on the PrP polymorphism at codons 136 (A/V), 154 (R/H) and 171 (R/H/Q).The resistance to prion infection is essentially associated with the presence of an arginine residue in position 171 of PrP and is particularly high in homozygous ARR/ARR animals. We described a new ELISA test (sandwich immunoassay) allowing a rapid, simple and cost effective characterization of the PrP polymorphism in position 171 on a plasma or serum sample. This test is based on the use of monoclonal antibody 2A11 which has the peculiar property of not recognizing PrP bearing a R residue in position 171 while it binds very efficiently PrP with an histidine (H) or a glutamine (Q) at the same position. The capture of PrP contained in the serum or plasma sample is achieved by another monoclonal antibody recognizing all PrP molecules independently of the polymorphism in position 171. This test unambiguously identifies animal bearing a ARR/ARR genotype. In addition it allows classifying with a excellent sensibility and specificity animal bearing a Q/Q or a H/Q genotype with regards to heterozygote R/Q or R/H. It is adapted for selection on a high throughput basis of animals resistant to TSEs.
AD J.M. Bilheude, S. Henaux: : Bio-Rad, R&D TSE, 3 Bd Raymond Poincaré, 92430 Marnes La Coquette, France; A. Brun, J.M. Torres: Center of Animal Health Investigation, National Institute of Agricultural Technology and Investigation, Valdeolmos, Madrid, Spain; N. Morel, J. Grassi: CEA, Service de Pharmacologie et Immunologie, CEA/Saclay, Gif-sur-Yvette, France; O. Andréoletti: UMR 1225 INRA ENVT IHAP, Ecole Nationale Vétérinaire de Toulouse, 23 chemin des capelles, 31076 Toulouse, France. E-mail: jmtorres@inia.es
SP englisch
PO Italien