NR AWCH
AU Blasche,T.; Schettler,E.; Balachandran,A.V.; Langenberg,J.; Miller,M.W.; Frölich,K.; Steinbach,F.
TI Rapid test applicability in detection of cervid PrP
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-08
PT Konferenz-Poster
AB Transmissible spongiform encephalopathies (TSE) occur in a number of animal species, (e.g. scrapie in sheep and goats and BSE in cattle). Chronic wasting disease (CWD) has emerged as an important TSE of captive and free-ranging cervids in North America, naturally affecting several species including mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginianus), Rocky Mountain elk (Cervus elaphus nelsoni) and moose (Alces alces shirasi). National surveys for TSE in cervids have recently been performed in Europe, the largest of them in Germany (n=6440). As successful surveillance and eradication strategies depend heavily on the tests used, we compared various commercially available rapid tests, originally developed for BSE and Scrapie screening, against prion protein (PrP) in cervids. Positive control samples (confirmed by PrP immunohistochemistry) originated from CWD cases from North America. Three ELISA-based tests, two western blots and one immunochromatographic assay by the companies Bio-Rad and Prionics were evaluated (additional data from other rapid tests will be presented soon). Brain samples from roe deer (Capreolus capreolus), red deer (Cervus elaphus elaphus) and fallow deer (Dama dama) sampled from different geographical regions in Germany were tested without using the proteinase K digestion step in PrP purification. Test procedures, especially those related to dilutions and other modifications to enhance sensitivity, were conducted according to the manufacturers. The western blot systems were used as reference to compare dilutions of PrP. All six tests evaluated were found to be suitable to detect PrP from cervid species tested. However, the analytical sensitivity and specificity demonstrated significant differences between the tests. We suggest the Bio-Rad Western Blot as reference as well as test of the choice for the detection of American cervid PrP giving highest values in quantitative and qualitative sensitivity. The Prionics LIA is best suited for detection of PrP from German cervid species. For future studies on cerivd PrP we recommend modification of the standard protocols, e.g. additional precipitation steps, triggering sensitivity to higer values.
AD T. Blasche, E. Schettler, K. Frölich, F. Steinbach: Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany; A. Balachandran: Canadian Food Inspection Agency, National Reference Laboratory, Ottawa, Canada; J. Langenberg: Wisconsin Department of Natural Resources, Madison, Wisconsin, USA; M.W. Miller: Colorado Division of Wildlife, Fort Collins, Colorado, USA. E-mail: f.steinbach@vla.defra.gsi.gov.uk
SP englisch
PO Italien