NR AWCK
AU Borthwick,E.B.; Brenn,A.; Groschup,M.H.; Talbot,R.; Hawkins,S.; Archibald,A.L.; Williams,J.L.
TI The search for differential gene expression in cattle spleen and white blood cells during BSE infection
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-09
PT Konferenz-Poster
AB Differential gene expression within peripheral tissues of BSE infected cattle could provide the means of identification for early BSE infection. The oral route of infection by the infected agent (PrPres) passes from the gastrointestinal system to the CNS via the lymporeticular system. Little is known of the effect on gene expression of the host organs during this early infection period; although there is some evidence of decreased levels of erythroid differentiation-related factor (EDRF) in the spleen and erythroid cells in the blood of TSE infected mice. Therefore there is a possibility of using differential gene expression in peripheral tissues as an early diagnostic test for BSE infection. In this project we are investigating the differential gene expression between normal and BSE infected cattle using RNA isolated from spleen and white blood cells. Spleen samples were collected from a DEFRA funded controlled BSE challenge project that was carried out at VLA in Weybridge using Holstein cattle. Blood samples were collected from a BSE challenge set up at Greifswald, Germany using Simmental cattle. Blood samples were collected during the BSE incubation and initially fractionated into PBMs (peripheral blood monocytes). At later time points individual white blood cells were separated into B cells, T cells and macrophages. RNA isolated from the spleen and PBM samples was used to probe bovine specific microarrays, using both dual colour array and the Affymetrix Bovine Genome Array. Analysis of the microarray experiments produced gene lists of significantly differentially expressed genes. Interestingly there are more down regulated genes during BSE infection compared to up regulated genes against age matched controls. These results require verification by quantitative PCR and classification into groups and families to enable a full understanding of the significance of these findings. It is possible that the outcome of this project could produce a panel of differential genes which may be used as part of an early diagnostic test for BSE infection.
AD E.B. Borthwick, R. Talbot, A.L. Archibald, J.L. Williams: Department of Genetics & Genomics, Roslin Institute, Roslin, Midlothian, Scotland, UK; A. Brenn, M. Groschup: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald, Insel Riems, Germany; S. Hawkins: VLA Weybridge, New Haw, Addlestone, Surrey,England, UK
SP englisch
PO Italien