NR AWCX
AU Cardone,F.; Berardi,V.A.; Valanzano,A.; De Pascalis,A.; Abdel-Haq,H.; Meyer,R.; Brown,P.; Pocchiari,M.
TI Application of high pressure-temperature treatments to remove TSE agents from biological products
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-02
PT Konferenz-Poster
AB The BSE epidemic and the resulting outbreak of variant CJD have been minimized by the adoption of several preventive measures but the recent discovery of 'home grown' cases of BSE, and the epidemic diffucion of chronic wasting disease in North America, call for the introduction of complementary strategies to ensure the safety of products of ruminant origin. High pressure-temperature (HPT) treatment is a product friendly technology able to reduce TSE infectivity by several orders of magnitude. Our goal was to develop practical conditions for the application of HPT treatment to remove TSE infectivity from biological products at risk. The first step was to study the relative contribution of single parameters of process (pressure, temperature and time) to TSE inactivation. We found a progressive reduction of PrPres in 263k scrapie spiked hot-dog treated at increasing pressures (600-1200 MPa/134 deg C/5 sec) or temperatures (115-134 deg C/1200MPa/5sec). Regarding the time of treatment, the maximal PrPres removal is already obtained after three minutes. The effect of HPT on low infectivity samples was also tested: more than 2.1 log LD50 of infectivity are cleared at 690 MPa/124 deg C, in line with the 2.8 log LD50 removed from a highly infectious preparation of 263k scrapie spiked hot dog. To verify the reliability of the process we performed multiple runs on identical aliquots of TSE spiked hot dog: we obtained comparable infectivity (>2.5 log LD50) and PrPres (2.0 log) removal. To assay the effect of the substrate we spiked different products with TSE infected brain paste and observed that the substrate do not affect significantly PrPres removal. As a whole, our results show that commercially practical conditions can be selected to remove TSE infectivity with minimal degradation of the product.
AD F. Cardone, V. Berardi, A. Valanzano, A. De Pascalis, H. Abdel-Haq: Instituto Superiore di Sanità, Dpt Cell Biology and Neurosciences, Rome, Italy; R. Meyer: Washington Farms, Tacoma, WA, USA; P. Brown: 7815 Exeter Road, Bethesda, MD, USA. E-mail: cardone@iss.it
SP englisch
PO Italien