NR AWCZ
AU Casalone,C.; Corona,C.; Iulini,B.; Porcario,C.; Caramelli,M.; Langeveld,J.P.M.; van Keulen,L.J.M.
TI Atypical BSE isolates in cattle: Immunohistochemical comparison between Dutch and Italian cases
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-08
PT Konferenz-Poster
AB Unusual isolates of BSE have been reported in the past two years in different European countries and Japan. Two variant forms of BSE have been reported based on biochemical analysis of the molecular mass and glycoform ratio of the pathological prion protein (PrPsc): i) a type with a lower molecular mass of the unglycosylated isoform and a distinct glycopattern of PrPres (L-type or BASE) and (ii) a type with higher molecular mass of the unglycosylated isoform and a distinct glycopattern of PrPres (H-type). From a pathological point of view, a careful description was only available for the two Italian cases (L-type) where immunohistochemical analysis revealed an unusual PrPsc deposition pattern characterized by the presence of amyloid plaques particularly in the white matter of olfactory bulb, frontal and parietal cortex. Here we report the results of a comparative immunohistochemical study between an H-type BSE case identified in the Netherlands and the two Italian L-type or BASE cases, using a panel of four different antibodies (9A2, 94B4, 12B2, F99/97.6.1). The antibodies used recognize different linear and conformational epitopes that are dispatched along the PrP sequence. The study conducted at the level of the obex and pons indicates that F99/97.6.1 better discriminates the two different phenotypes.
AD C. Casalone, C. Corona, B. Iulini, C. Porcario, M. Caramelli: CEA-Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy; J.P.M. Langeveld, L.J.M. van Keulen: Department of Bacteriology and TSEs, Central Institute for Animal Disease Control (CIDC), 8023 AA Lelystad, The Netherlands. E-mail: cristina.casalone@izsto.it
SP englisch
PO Italien