NR AWDA
AU Catalano,D.; Amedeo,S.; Baldi,A.; Bianco,P.; Cheli,F.; Cornaglia,E.; Dondo,A.; Macri,B.; Marino,F.; Mioletti,S.; Rosati,S.; Scaglione,F.E.; Valenza,F.; Capucchio,M.T.
TI PrP-plasminogen interaction in health and mastitic bovine mammary gland: preliminary data
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-05
PT Konferenz-Poster
AB Prion diseases involve conversion of a physiologic protein PrPc to the pathological isoform, PrPsc. Plasminogen (PG) has been shown to interact with PrPsc. The plasminogen activator system (Pas) is the most important in degrading extracellular proteins. PG crosses directly from blood to milk and it is converted to plasmin during lactation and mammary gland involution. The aim of this study is to evaluate the possible interactions between PrP/Pas on mammary tissue samples of health (n°0) and mastitic (n°50) cows collected in different range of lactation (0-1.5; 1.3-3; 3-7; 7-10; more than 10 months in lactation). The Pas in milk and mammary tissues was also investigated. Immunohistochemical and immunogold post-embedding techniques using different antibodies (12F10 (CEA) anti PrP Mab; 7336 (AbCAM) anti PG Pab and a new anti uPAR Pab) were performed on fixed samples. Bacteriological investigations were performed too. The milk fractions were analysed using a colorimetric assay for plasmin, PG and plasminogen activator. All methods revealed increased levels of Pas with advancing lactation and with mastitis. Any significative variations in the expression of uPAR were observed relatively to different isolated bacteria. The study on tissues showed the presence of PrPc anchored to the cell surface of the plasma membrane and into the cells. No PG immunoreactivity was observed. uPAR expression was limited to the cytoplasmic structures. Further studies are necessary, but it seems that there is no interaction, at mammary gland level, between PrPc and different components of Pas (PG, uPAR) also during mastitis, condition associated to an increased PG presence in the gland. This work was supported by funds of MURST 2003 and Piemonte Region 2003-2004.
AD Faculty of Veterinary Medicine, University of Torino, 1Milano,4Messina. 2ASL11 Vercelli; 3IZS Torino; Italy. E-mail: mariateresa.capucchio@unito.it
SP englisch
PO Italien