NR AWDC
AU Cawthraw,S.; Saunders,G.C.; Griffiths,P.C.; Tout,A.C.; Wiener,P.; Woolliams,J.A.; Williams,J.L.; Windl,O.
TI PrP gene polymorphisms in BARB BSE cattle in Great Britain
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions GEN-08
PT Konferenz-Poster
AB The aim of this study was to establish whether polymorphisms in the open reading frame (ORF) or promoter regions of the PrP gene from cattle born-after-the-reinforced ban (BARB) are associated with increased susceptibility to BSE disease. These cattle, born after August 1996, are diverse in breed, age and geographical location and because of regulations on feed ingredients should not have been orally exposed to the BSE agent in contaminated feed. The ORF (1.1 kb) of the PrP gene from 101 BARB BSE cases and the promoter region (5 kb including exon 1 and 2) from 49 BARB BSE cases were sequenced. In addition, when suitable animals could be located matched control animals were also sequenced. Within the ORF, four silent single nucleotide polymorphisms (SNPs) were detected corresponding to codon positions L23 (leucine), Q78 (glutamine), P113 (proline) and N192 (asparagine). One non-conservative polymorphism was found in the which affected the number of octapeptide repeats in the PrP N-terminal region. Genotypes with 6:6, 6:5, 5:5 and 6:7 repeats were detected. For the PrP promoter region, 51 common polymorphisms were identified including two major indels (insertions/deletions) of 23bp, located upstream of exon 1, and 12 bp, located in intron 1. Five main promoter haplotypes were determined from the promoter sequence data generated. No polymorphisms identified in the PrP gene coding region or promoter region were found to be associated with increased susceptibility to BSE in the BARB cases when compared to controls. However, within the BSE BARB group, a homozygous SNP in the ORF was found to be putatively associated with an absence of clinical symptoms (p < 0.05), but this may be explained by breed and is being investigated further. This project was funded by Defra, UK
AD S. Cawthraw, G.C. Saunders, P.C. Griffiths, A.C. Tout, O. Windl: Department of TSE Molecular Biology, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK; P. Wiener, J.A. Woolliams, J.L. Williams: Division of Genomics and Bioinformatics, Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, UK; J.L. Williams: current address: Parco Tecnologico Padano, Via Einstein, 2690 Lodi, Italy.
SP englisch
PO Italien