NR AWDE
AU Cervenakova,L.
TI Variant Creutzfeldt-Jakob disease and blood infectivity in animal models
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Oral sessions ORAL-53
PT Konferenz-Vortrag
AB Patients with variant Creutzfeldt-Jakob disease (vCJD) accumulate infectivity and disease-associated prion protein (PrPTSE) in lymphoreticular tissues more extensively than patients with classical CJD. These findings suggest that the blood of vCJD patients may also carry higher levels of infectivity. Three instances of secondary transmission of infection associated with blood transfusion have recently been reported in the UK, increasing the concern about blood safety. We have shown that during the clinical phase of the disease, buffy coat from mice inoculated with mouse-adapted human strains of Gerstmann-Sträussler-Scheinker disease (GSS) had a higher level of infectivity than buffy coat from mice inoculated with vCJD. In contrast, during the preclinical stage of disease, both buffy coat and plasma from vCJD-inoculated mice had higher levels of infectivity than mice inoculated with GSS. We also showed that infection is efficiently transmitted by i.c. and i.v. routes for buffy coat and platelet poor plasma. Here, we present data on rates of transmission by i.v. inoculation of whole blood from animals infected with vCJD, and discuss the results in connection with other investigations of TSE transmission through blood or blood components using a variety of animal models.
AD Transmissible Diseases Department, American Red Cross Holland Laboratory, Rockville, Maryland, USA. E-mail: cervenakl@usa.redcross.org
SP englisch
PO Italien