NR AWDI
AU Chianini,F.; Di Bari,M.A.; Nonno,R.; Hamilton,S.; Finlayson,J.; Eaton,S.; Steele,P.; Rodger,S.; Dalgleish,M.P.; Jeffrey,M.J.; Reid,H.W.; Agrimi,U.
TI Bank voles are useful bioassays for ARQ/ARQ Suffolk scrapie infection
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-09
PT Konferenz-Poster
AB Scrapie is a transmissible spongiform encephalopathy (TSE) affecting sheep, goats and moufflon. The influence of genetic susceptibility in sheep is well described and the occurrence of natural and experimental scrapie has been linked to polymorphisms at codons 136, 154 and 171 of the PrP gene. The effect of such polymorphisms is also dependent on breed and in Suffolk sheep the ARQ/ARQ genotype is considered the most susceptible whilst the ARR/ARR genotype is most resistant to natural disease. The determination of levels of infectivity in tissues from scrapie affected sheep is fundamental to understand the pathogenesis, and the transmissibility of the disease. The most sensitive indicator of Suffolk scrapie infectivity is probably the parenteral challenge of ARQ/ARQ sheep. Such a system is however cumbersome and expensive. Detection of infection in laboratory mice is more rapid and economical, but is generally less sensitive, particularly in the case of primary isolation from Suffolk sheep. Recently, the bank vole (Clethrionomys glareolus) has been shown to be highly sensitive to different sources of natural scrapie. After intracerebral inoculation, bank voles succumb to disease following a short incubation time (around 200 days post inoculation) and also show high attack rates. With this study we established that voles are very susceptible to inoculation from ARQ/ARQ Suffolk scrapie, and that they succumb in 175 +/- 18 days after inoculation of 0.02 ml of brain homogenate.
AD F. Chianini, S. Hamilton, J. Finlayson, S. Eaton, P. Steele, S. Rodger, M.P. Dalgleish, H.W. Reid: Moredun Research Institute, Edinburgh, UK; M.A. Di Bari, R. Nonno, U. Agrimi: Istituto Superiore di Sanita', Roma, Italy; M. Jeffrey: Veterinary Laboratory Agency, Lasswade, Edinburgh, UK. E-mail: francesca.chianini@moredun.ac.uk
SP englisch
PO Italien