NR AWDM
AU Colussi,S.; Rasero,R.; Santagada,G.; Maniaci,M.G.; Sacchi,P.; Laurenza,C.; Riina,M.V.; Peletto,S.; Trisorio,S.; Ingravalle,F.; Maurella,C.; Caramelli,M.; Acutis,P.L.
TI Genetic variability of the PrP gene in goat breeds from Northern and Southern Italy
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions GEN-09
PT Konferenz-Poster
AB Prion protein (PrP) gene polymorphism in Italian sheep breeds have been well investigated, whereas little is known about goats. Aim of this work is to determine the variability of PrP gene in goat breeds from Piedmont and Basilicata, a Northern and a Southern Italian region respectively. Blood samples were collected from 300 goats of the four most common breeds in Piedmont region and from 216 goats of 10 breeds from Basilicata. PrP gene polymorphisms were detected by direct DNA sequencing on both strands of the PCR products. Thirteen PrP polymorphisms were identified: G37V, T110P, G127S, M137I, I142M, I142T, H143R, R154H, P168Q, T194P, R211Q, Q222K, S240P (the dimorphisms I142T and T194P are novel). This gave rise to 14 protein variants (nine in the Northern breeds and 12 in the Southern breeds). The allele with the mutation S240P only has been found at the highest frequency in both groups (38.8% and 54.2% in Northern and Southern goats respectively). Mutations G37V, M137I, M142T, H143R and T194P have not been detected in Northern goats while G127S and R211Q have not been found in Southern breeds. For G37V, H143R, 2 2 G127S and R211Q the associations are statistically significant ( =23.95, p-value=<0.05; =20.48, 2 2 p-value=<0.05; =10.27, p-value=<0.05; =45.51, p-value=<0.05 respectively). I142M is present at higher frequency in Northern goats (12.3% vs 0.8%) (2=39.83, p-value=<0.05). Q222K (2.7% vs 14.6%) and R154H (4% vs 7.6%) are significantly associated with breeds from the south (2=48.21, p-value=<0.05; 2=5.68, p-value=<0.05, respectively). The present study revealed a high variation in the caprine PrP gene and clear differences between Northern and Southern breeds, with mutations exclusively or significantly associated with one group or the other.
AD S. Colussi, M.G. Maniaci, M.V. Riuina, S. Peletto, S. Trisorio, F. Ingravalle, C. Maurella, M. Caramelli, P.L. Acutis: Istituto Zooprofilattico del Piemonte, Turin, CEA, Italy; R. Rasero, P. Sacchi: Veterinary Medicine University, Turin, Italy; G. Santagada, C. Laurenza: Istituto Zooprofilattico della Puglia e della Basilicata, Matera, Italy. E-mail: silvia.colussi@libero.it
SP englisch
PO Italien