NR AWDO
AU Corona,C.; Martucci,F.; Iulini,B.; Mazza,M.; Acutis,P.L.; Porcario,C.; Pezzolato,M.; Manea,B.; Maroni,A.; Barocci,S.; Bozzetta,E.; Caramelli,M.; Casalone,C.
TI Could oestrus ovis act as vector for scrapie?
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-10
PT Konferenz-Poster
AB Animal prion infections, such as scrapie and CWD, have shown a pattern of horizontal transmission in farm conditions. Previous studies on different ecto-parasites affecting domestic and wild ruminants, demonstrated the presence of PrPsc in fly larvae and pupae, speculating on the possible role of such parasites as vectors and/or reservoirs for TSE's. Oestrosis, the nasal myasis of sheep and goats, is caused by the larvae of Oestrus ovis that develop from the first to the third stage larva in nasal cavities and frontal sinuses of the host. To investigate the possible role of Oestrus ovis in the pathogenesis of scrapie infection, a large number of larvae was collected from the nasal cavities of positive and negative scrapie cases to be examined by Western blot. Furthermore, a panel of different organs including CNS, third eyelid and olfactory mucosa at the level of labyrinthus ethmoidalis was analysed by immunohistochemistry and Western blot methods. Here we report the presence of PrPsc in the larvae of Oestrus ovis found in three natural scrapie affected sheep coming from two different Italian outbreaks. Interestingly a linkage between positive larvae and positive olfactory mucosa was observed.
AD C. Corona, F. Martucci, B. Iulini, M. Mazza, P.L. Acutis, C. Porcario, M. Pezzolato, B. Manea, E. Bozzetta, M. Caramelli, C. Casalone: CEA-Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy; A. Maroni: Dipartimento per la sanità pubblica veterinaria la nutrizione e la sicurezza degli alimenti- Ministero della salute, Rome Italy; S. Barocci: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini 1, 06126 Perugia Italy. E-mail: cristiano.corona@izsto.it
SP englisch
PO Italien